Part:BBa_K5302017:Design

pBBR-OmpA-8IIU

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3931
  Illegal EcoRI site found at 4301
  Illegal XbaI site found at 3140
  Illegal PstI site found at 1967
  Illegal PstI site found at 3128
  Illegal PstI site found at 4033
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3931
  Illegal EcoRI site found at 4301
  Illegal PstI site found at 1967
  Illegal PstI site found at 3128
  Illegal PstI site found at 4033
  Illegal NotI site found at 1008
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3931
  Illegal EcoRI site found at 4301
  Illegal BglII site found at 1754
  Illegal BamHI site found at 3146
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3931
  Illegal EcoRI site found at 4301
  Illegal XbaI site found at 3140
  Illegal PstI site found at 1967
  Illegal PstI site found at 3128
  Illegal PstI site found at 4033
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3931
  Illegal EcoRI site found at 4301
  Illegal XbaI site found at 3140
  Illegal PstI site found at 1967
  Illegal PstI site found at 3128
  Illegal PstI site found at 4033
  Illegal NgoMIV site found at 2418
  Illegal NgoMIV site found at 2701
  Illegal NgoMIV site found at 4959
  Illegal AgeI site found at 4799
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1330
  Illegal SapI.rc site found at 2267
  Illegal SapI.rc site found at 2477
  

Design Notes

Please be advised that all listed genes have undergone codon optimization for our chassis strain, Escherichia coli Nissle 1917 (EcN). For those considering alternative chassis organisms, it is recommended to reassess the necessity of codon optimization.

Source

Source Organism of 8IIU: Homo sapiens. OmpA (Outer membrane protein A) from Escherichia coli str. K-12 substr. MG1655.